B003: Leica Super-Resolution Microscope TCS SP8 STED [ バイオ解析 | Bioanalysis ]
Detail information
| Machine info |
Maker: Leica Spec: ・Live-cell confocal imaging ・Gated STED 2D super-resolution imaging with 50-nm (xy) resolutions ・5 laser lines (458, 476, 488, 514, 633 nm) and white light laser (470 – 670 nm) ・1 to 3-color STED imaging by 2 depletion laser lines (592, 660 nm) Installation Year: AY2009 [Detailed Specs] ・Motorized confocal microscope SP8 (based on Leica DMI 6000 CS) ・Adaptive Focus Control unit (maintains the desired focal plane for long-term imaging) ・Lasers Ar (458, 476, 488, 514 nm), HeNe (633 nm) White light laser: continuous wave laser in the range of 470-670nm; AOTF allows to choose up to 8 different wavelengths simultaneously; pulsed laser system with a repetition rate of 80 MHz Continuous wave depletion laser (592, 660 nm) for STED Ti:Sapphire pulsed laser (Coherent Chameleon) ・Detectors 2ch PMT fluorescence detectors 2ch high-sensitive hybrid GaAsP detector HyD; time gated detection allows improvement of STED lateral (xy) resolution to <50 nm by suppressing short-lived signals at the edge of focal spot 1ch Transmitted light detector ・Scanner FOV scanner with dual scanning galvanometer mirrors allows for either high speed scanning 7 images/sec at 512 x 512 or high pixel density scanning 8k x 8k (64 megapixels) per channel. ・Motorized XY stage and Z galvo stage for high-precision Z-sectioning ・DIC unit ・Objectives Dry lens HC PL APO CS 10x/0.40 Oil lens HCX PL APO CS 40x/1.25-0.75, HCX PL APO CS 63x/1.40-0.60, HC PL APO 100x/1.40 STED WHITE Water lens HCX PL APO 63x/1.20 W CORR CS ・Cell culture chamber with temperature and CO2 Control (Tokai Hit) ・Deconvolution software with STED option (Huygens) Location: 総合研究1号館別館 301 | Research Bldg. No.1 Annex Rm. 301 : 藤原敬宏/Takahiro Fujiwara, tfujiwara@icems.kyoto-u.ac.jp Machine no: B003 |
|---|---|
| Please login to download related documents. | |
| Support information | Please login to download related documents. |