ImageName
B001: Nikon Fast-Scan Confocal Microscope A1R
  • ・Live-cell confocal imaging
  • ・High-speed confocal imaging by resonant scanner and piezo z stage scanner
  • ・4 laser lines (405, 488, 561, 640 nm)
  • ・Spectral unmixing by 32ch spectral detector unit
  • Installation Year: 2007
[ Detail ]
  • ・Eclipse Ti-E, Inverted
  • ・Ti PerfectFocus Unit (maintains the desired focal plane for long-term live cell imaging)
  • ・Motorized XY Stage
  • ・Piezo Z Scanner
  • ・Epi Fluorescence Unit (Intensilight Precentered Fiber Illumination)
  • ・DIC Unit
  • ・Lasers 405, 488, 561, 640 nm
  • ・Objectives CFI Plan Apo 10x, CFI Plan Apo VC 20x, CFI S Plan Fluor ELWD 20xC, CFI Plan Apo Lambda D 40x, CFI Plan Apo VC 60x oil, CFI Plan Apo VC 60x water
  • ・Epi Fluorescence Filter Set DAPI、FITC、TRITC
  • ・High-speed Resonant Scanner (7.8 kHz) for High-speed Imaging at 30 fps (512 x 512 pixels) and 420 fps (512 x 32 pixels)
  • ・2 x GaAsP Detectors (for 488 and 561 nm channels), 2 x PMT Detectors (for 405 and 640 nm channels), 1 x PMT Detector (for DIC)
  • ・1 x 32 channels Spectral Detector Unit for Spectral Imaging at 1.67 fps (512 x 512 pixels) and 24 fps (512 x 32 pixels)
  • ・Fluorescence Filters 450/50, 482/35, 525/50, 540/30, 595/50, 700/75
  • ・Cell Culture Chamber with Temperature and CO2 Control (Tokai Hit)
B002: Olympus Confocal Microscope FV-1000 with Super-Resolution Unit
  • ・Live-cell confocal imaging
  • ・FV-OSR 2D super-resolution imaging with 120-nm (xy) resolutions
  • ・6 laser lines (405, 440, 488, 515, 559, 635 nm)
  • Installation Year: 2007
[ Detail ]
  • ・Motorized Inverted Microscope with Automatic Focus Correction Unit IX81ZDC
  • ・Motorized XY stage
  • ・Epi Fluorescence Unit (Mercury Lamp Illumination)
  • ・DIC Unit
  • ・Lasers 405, 440, 488, 515, 559, 635 nm
  • ・Objectives UPLSAPO 10x, UPLSAPO 20x, UPLSAPO 40x, UPLSAPO 60x OIL, UPLSAPO 100x OIL, UPLSAPO30XS Silicone Immersion
  • ・Epi Fluorescence Filter Set DAPI, FITC, TRITC
  • ・4 x PMT Detectors
  • ・GaAsP Detector
  • ・Super Resolution Unit and Software FV-OSR
  • ・Cell Culture Chamber with Temperature and CO2 Control (Tokai Hit)
B003: Leica Super-Resolution Microscope TCS SP8 STED
  • ・Live-cell confocal imaging
  • ・Gated STED 2D super-resolution imaging with 50-nm (xy) resolutions
  • ・5 laser lines (458, 476, 488, 514, 633 nm) and white light laser (470 – 670 nm)
  • ・1 to 3-color STED imaging by 2 depletion laser lines (592, 660 nm)
  • Installation Year: 2009
[ Detail ]
  • ・Motorized confocal microscope SP8 (based on Leica DMI 6000 CS)
  • ・Adaptive Focus Control unit (maintains the desired focal plane for long-term imaging)
  • ・Lasers Ar (458, 476, 488, 514 nm), HeNe (633 nm)
  • White light laser: continuous wave laser in the range of 470-670nm; AOTF allows to choose up to 8 different wavelengths simultaneously; pulsed laser system with a repetition rate of 80 MHz
  • Continuous wave depletion laser (592, 660 nm) for STED
  • Ti:Sapphire pulsed laser (Coherent Chameleon)
  • ・Detectors
  • 2ch PMT fluorescence detectors
  • 2ch high-sensitive hybrid GaAsP detector HyD; time gated detection allows improvement of STED lateral (xy) resolution to <50 nm by suppressing short-lived signals at the edge of focal spot
  • 1ch Transmitted light detector
  • ・Scanner
  • FOV scanner with dual scanning galvanometer mirrors allows for either high speed scanning 7 images/sec at 512 x 512 or high pixel density scanning 8k x 8k (64 megapixels) per channel.
  • ・Motorized XY stage and Z galvo stage for high-precision Z-sectioning
  • ・DIC unit
  • ・Objectives
  • Dry lens HC PL APO CS 10x/0.40
  • Oil lens HCX PL APO CS 40x/1.25-0.75, HCX PL APO CS 63x/1.40-0.60, HC PL APO 100x/1.40 STED WHITE
  • Water lens HCX PL APO 63x/1.20 W CORR CS
  • ・Cell culture chamber with temperature and CO2 Control (Tokai Hit)
  • ・Deconvolution software with STED option (Huygens)
B004: Zeiss Confocal FCCS Microscope LSM780
  • ・Long-term live-cell confocal imaging
  • ・7 laser lines (405, 440, 458, 488, 514, 561, 633 nm)
  • ・Spectral unmixing by 32ch GaAsP detector array
  • ・Molecular interaction detection by FCS/FCCS (ConfoCor3 with APD detector)
  • Installation Year: 2009
[ Detail ]
  • ・Motorized inverted microscope AxioObserver.Z1
  • ・Lasers 405, 440, 458, 488, 514, 561, 633 nm
  • ・Detectors
  • 34ch QUASAR GaAsP spectral detector
  • 1ch Transmitted light detector
  • 2ch External detector for multiphoton (for GFP and RFP)
  • 2ch APD detector for FCS/FCCS
  • ・Automatic focus correction unit (Definite Focus)
  • ・Motorized XY stage
  • ・Wide-field fluorescence unit (X-Cite fiber-coupled light source)
  • ・DIC unit
  • ・Objectives Plan-Apochromat10x/0.45, Plan-Apochromat 20x/0.80, LCI Plan-Neofluor 25xImm/0.80, C-Apochromat 40xWater/1.20, Plan-Apochromat 63xOil/1.40
  • ・Cell culture unit (Incubation chamber + Heater + CO2 supply)
  • ・Narishige micromanipulator
B005: Zeiss Super-Resolution Microscope LSM880 Airyscan
  • ・Long-term live-cell confocal/super-resolution imaging
  • ・6 laser lines (405, 458, 488, 514, 561, 633 nm)
  • ・Airyscan super-resolution imaging with 120-nm (xy) and 350-nm (z) resolutions
  • ・Airyscan fast mode with 4x acquisition speed
  • ・Spectral unmixing by 32ch GaAsP detector array
  • Installation Year: 2015
[ Detail ]
  • ・Motorized inverted microscope AxioObserver.Z1
  • ・Lasers 405, 458, 488, 514, 561, 633 nm
  • ・Detectors
  • 34ch QUASAR GaAsP spectral detector
  • 1ch  Transmitted light detector
  • Airyscan detector with fast acquisition mode (4x faster)
  • ・Automatic focus correction unit (Definite Focus)
  • ・Motorized XY stage
  • ・Piezo Z stage
  • ・Wide-field fluorescence unit (HXP120V fiber-coupled light source)
  • ・DIC unit
  • ・Objectives Plan-Apochromat10x/0.45, Plan-Apochromat 20x/0.80, LCI Plan-Neofluor 25xImm/0.80, Plan-Apochromat 40xOil/1.30, Plan-Apochromat 40xOil/1.40, Plan-Apochromat 63xOil/1.40 for super-resolution, Plan-Apochromat 100xOil/1.46 for super-resolution
  • ・Cell culture unit (Incubation chamber + Heater + CO2 supply)
B006: Zeiss Confocal Microscope LSM710
  • ・Long-term live-cell confocal imaging
  • ・5 laser lines (458, 488, 514, 561, 633 nm)
  • ・Tiling/stitching for multiple fields
  • Installation Year: 2009
[ Detail ]
  • ・Motorized inverted microscope AxioObserver.Z1
  • ・Lasers 458, 488, 514, 561, 633 nm
  • ・Detectors
  • 2ch QUASAR spectral detector
  • 2ch LSM BiG GaAsP detector (for GFP and RFP)
  • 1ch Transmitted light detector
  • ・Automatic focus correction unit (Definite Focus)
  • ・Motorized XY stage
  • ・Wide-field fluorescence unit (X-Cite fiber-coupled light source)
  • ・DIC unit
  • ・Objectives Plan-Apochromat10x/0.45, LD LCI Plan-Apochromat25xImm/0.80, Plan-Apochromat 40xOil/1.40, LD C-Apochromat 40xWater/1.10, Plan-Apochromat 100xOil/1.46
  • ・Cell culture unit (Incubation chamber + Heater + CO2 supply)
B007: Zeiss Multiphoton Fast Super-Resolution Microscope LSM980NLO Airyscan2
  • ・Long-term live-cell confocal/super-resolution/2-photon imaging
  • ・7 laser lines (405, 445, 488, 561, 639, Chameleon Ultra II 680-1080 nm)
  • ・Airyscan2 super-resolution imaging with 120-nm (xy) and 350-nm (z) resolutions
  • ・Airyscan2 fast and gentle multiplex mode with 4x and 8x acquisition speeds
  • ・Spectral unmixing by 32ch GaAsP detector array
  • Installation Year: 2019
[ Detail ]
  • ・Motorized inverted microscope AxioObserver.7
  • ・Lasers
  • 405, 445, 488, 514, 561, 639 nm, Coherent Chameleon Ultra II (680-1080 nm)
  • ・Detectors
  • 34ch  QUASAR GaAsP spectral detector
  • 1ch  Transmitted light detector
  • Airyscan 2 detector with multiplex (4x and 8x) acquisition modes
  • ・Automatic focus correction unit (Definite Focus.2)
  • ・Motorized XY stage
  • ・Piezo Z stage
  • ・Wide-field fluorescence unit (HXP120V fiber-coupled light source)
  • ・DIC unit
  • ・Objectives
  • Plan-Apochromat10x/0.45
  • Plan-Apochromat 20x/0.80
  • Plan-Apochromat 63xOil/1.40 for super-resolution
  • alpha Plan-Apochromat 63xOil/1.46 for super-resolution
  • ・Cell culture unit (Incubation chamber + Heater + CO2 supply)
  • ・Arivis Vision 4D
B008: Andor Multimodal Fast Confocal Microscope System Dragonfly
  • ・Spinning disk confocal imaging with uniform Borealis illumination
  • ・Fast live-cell imaging up to 400 fps and 3D imaging for large volume samples
  • ・4 laser lines (405, 488, 561, 637 nm)
  • ・2 high-sensitive cameras (iXon Ultra 888 EMCCD and Zyla 4.2 Plus sCMOS)
  • ・Mosaic3 photostimulation unit with 405 nm laser
  • Installation Year: 2019
[ Detail ]
  • ・Dragonfly 502
  • Spinning disk with 2 pinhole diameters (25 and 40 microns), Borealis illumination, Laser-illuminated widefield mode, 2 camera ports, Camera magnification (3-position motorized), Illumination zoom, GPU-accelerated deconvolution in parallel with acquisition, Imaris Core
  • ・Motorized inverted microscope Nikon Ti2-E
  • ・Lasers 405 nm/100 mW, 488 nm/150 mW, 561 nm/100 mW, 637 nm/140 mW
  • ・Cameras
  • iXon Ultra EMCCD
  • Zyla 4.2 Plus sCMOS 
  • ・Automatic focus correction unit (Perfect Focus)
  • ・ASI motorized XY stage and stage piezo (500-micron range)
  • ・Objectives
  • CFI Plan Apochromat Lambda 10X Dry NA 0.45 WD 4.00
  • CFI Plan Fluor 20X MImm (Oil/Glycerin/Water) NA 0.75 WD 0.35-0.33
  • CFI Plan Apochromat Lambda S 25X Silicone NA 1.05 WD 0.55
  • CFI Plan Apochromat Lambda S 40X Silicone NA 1.25 WD 0.30
  • CFI Plan Apochromat VC 60XC Water NA 1.20 WD 0.31-0.28
  • CFI Apochromat TIRF 60XC Oil NA 1.49 WD 0.13-0.07 (37oC)
  • CFI Apochromat TIRF 100XC Oil NA 1.49 WD 0.15-0.09 (37oC)
  • CFI Plan Apo Lambda 60X Oil NA 1.40 WD 0.13
  • CFI Plan Apo VC 100X Oil NA 1.40 WD 0.13
  • ・Tokai Hit stage top incubator
  • ・Mosaic3 photostimulation unit with 405 nm/450 mW laser
B009: Zeiss Super-Resolution Microscope Elyra7 with Lattice SIM^2
  • ・Live-cell super-resolution imaging up to 255 fps
  • ・4 laser lines (405, 488, 561, 642 nm)
  • ・Light efficient lattice structured illumination imaging and advanced deconvolution (Lattice SIM^2) with 60-nm (xy) and 200-nm (z) resolutions
  • ・SIM^2 Apotome mode for fast optical sectioning
  • ・Simultaneous dual-color imaging by 2 high-sensitive cameras (PCO edge 4.2 sCMOS)
  • Installation Year: 2021
[ Detail ]
  • ・Motorized inverted microscope AxioObserver.7
  • ・Lasers
  • 405 nm/50 mW, 488 nm/100 mW, 561 nm/100 mW, 642 nm/150 mW
  • ・Cameras
  • PCO edge 4.2 sCMOS x2 with Duolink module
  • ・Automatic focus correction unit (Definite Focus.2)
  • ・Motorized Z drive
  • ・XY (130 x 100 mm) & Z (100 µm) piezo scanning stage
  • ・Wide-field fluorescence unit (HXP120V fiber-coupled light source)
  • ・Objectives
  • EC Plan-Neofluar 10x/0.3
  • LD LCI Plan-Apochromat 25x/0.8 Imm Corr
  • Plan-Apochromat 40x Oil/1.40
  • alpha Plan-Apochromat 100x Oil/1.46
  • ・Cell culture unit (Incubation chamber + Heater + CO2 supply)
B101: BD FACSCanto II Flow Cytometer
  • ・two lasers (L1: Blue 488 nm, L2: Red 633 nm)
  • ・six colors (See the following config for the details.)
  • ・FACSDiva 8 software
  • ・High Throughput Sampler (HTS), available for a 98- or 384-well microplate
  • Installation Year: 2008
[ Detail ]
  • Blue A: PE-Cy7 (735 LP, 780/60)
  • Blue B: PerCP-Cy5-5 (655LP, 670LP)
  • Blue C: (610LP)
  • Blue D: PE (556LP, 585/42)
  • Blue E: FITC (502LP, 530/30)
  • Blue F: SSC (488/10)
  • Red A: APC-Cy7 (735LP, 780/60)
  • Red B: (685LP)
  • Red C: APC (660/20)
B102: BD FACS Aria II SORP Cell Sorter
  • ・4-lasers (L1: Blue 488 nm, L2: Red 640 nm, L3: UV 355 nm, L4: Green 532 nm)
  • ・13 colors (See the following config for the details.)
  • ・FACSDiva 8 software
  • ・Plate sorting
  • ・Cooling system
  • Installation Year: 2009
[ Detail ]
  • Blue A: PE-Cy7 (750 LP, 780/60)
  • Blue B: PerCP-Cy5-5 (685LP, 710/50)
  • Blue C: PE-Cy5 (635LP, 660/20)
  • Blue D: PE-Texas Red (600LP, 610/20)
  • Blue E: PE (550LP, 575/25)
  • Blue F: FITC (505LP, 525/50)
  • Red A: APC-Cy7 (750LP, 780/60)
  • Red B: Alexa Fluor 700 (690LP, 730/45)
  • Red C: APC (660/20)
  • UV A: Hoechst Red (635LP, 670/50)
  • UV B: Hoechst Blue (450/40)
  • Green A: Grn PE-Cy7 (750LP, 780/60)
  • Green B: Grn PE (575/25)
B104: ProteinSimple Capillary Immnoblotting Jess System
  • ・Capillary chemiluminescent and fluorescent Western blotting
  • ・2-40 kDa, 12-230 KDa or 66-440 kDa/8 x 13 or 8 x 25 capillary cartridge available
  • Installation Year: 2020
[ Detail ]
B105: Applied Biosystems 4 capillaries DNA sequencer SeqStudio Genetic Analyzer
  • ・Sanger sequencing
  • ・4-capillary
  • ・Thermo Fisher Connect compatible (available for remote-monitoring from home)
  • Installation Year: 2020
B106: Applied Biosystems RT-PCR system QuantStudio 3
  • ・three independent temperature zones for precise temperature control and optimization
  • ・compatible with the standard 96 well (MicroAmp Optical 96-well Reaction Plate (Cat. no. N80 10560) or MicroAmp Optical 8-Tube strip (0.2 mL, Cat. no. 4316567))
  • ・Thermo Fisher Connect compatible (available for remote-monitoring from home)
  • Installation Year: 2021
M001: JEOL Field Emission Transmission Electron Microscope (FE-TEM) JEM-2200FS+JED2300T
  • ・local state analysis such as morphological observation and structural analysis in nano-scale
  • ・Elemental analysis for the nano regions by EDX
  • ・High-contrast observation with an omega-type energy filter
  • ・Low temperature, high temperature sample holder
  • ・resolution lattice 0.05nm, point 0.14nm @200kV
  • ・TEM(BF,DF), SAED, STEM(BF,HAADF), EDX(point, line, mapping), EELS
  • Installation Year: 2009
M002: JEOL Field Emission Scanning Electron Microscope (FE-SEM) JSM-7500F
  • ・Shape observation and composition analysis at the nano- micro-scale
  • ・resolution 1.0nm(15kV), 1.4nm(1kV)
  • ・acc. voltage 0.1kV~30kV
  • ・magnification x25~x1,000,000
  • ・Method low acceleration voltage mode (Gentlebeam), Energy filter(ganma-filter)
  • Installation Year: 2008
M003: Rigaku X-ray Absorption Spectrometer R-XAS Looper
  • ・Analysis of the elemental composition and structure in a sample
  • ・Max 3kW
  • ・Non-destructive analysis
  • ・Elemental selectivity
  • ・Local structure analysis in a specific element
  • Installation Year: 2008
M004: Bruker Nuclear Magnet Resonance Spectrometer (NMR) AVANCE III 500USP
  • ・Analysis of molecular structure of organic molecules in solutions
  • resonance frequency(1H) 500 MHz
  • ・Probe 1H/19F & 31P~15N 5mm Auto tuning mode
  • ・Autosampler(max 24sample)
  • ・Temperature-variable measurement -20℃~+100℃
  • Installation Year: 2009
M006: JEOL Nuclear Magnet Resonance Spectrometer (NMR) JNM-ECZ600R/M3
  • 600 MHz solid NMR system(with 24-sample ASC)
  • CZR-type solid NMR spectrometer (with middle power amplifier)
  • 1 mm HX MAS/wide temperature range 3.2 mm HX MAS/8 mm HX MAS/3.2 mm AutoMAS/GR (High gradient magnetic field, sample tube for solid)/5 mmFG/ROYALHFX
  • Installation Year: 2019
M007: Hitachi High-Tech Schottky Scanning Electron Microscope SU5000
  • ・Shape observation and composition analysis at the nano- to micro-scale
  • ・resolution 1.2 nm(30kV), 3.0 nm(1kV)
  • ・acc. voltage 0.5 kV~30 kV
  • ・magnification x 25 ~ x 600,000
  • ・Energy Dispersive X-ray Spectrometer(EDS)、large area observation
  • Installation Year: 2014
M103: BELSORP-MINI X Absorption Measurement Instrument
  • ・Gas Adsorption/Desorption (N2, CO2, O2, Ar)
  • ・77 K (Liquid Nitrogen), 0~40 ˚C
  • ・BET surface area
  • Installation Year: 2021
M104A: BELSORP-MAX X-A Absorption Measurement Instrument
  • ・Gas Adsorption/Desorption (He, N2, O2, CO2, Ar)
  • ・H2O, Organic liquids available
  • ・77 K (Liquid Nitrogen), 0~40 ˚C
  • ・BET surface area
  • ・Measurement in extremely low pressure
  • ・Pore distribution analysis
  • Installation Year: 2010, 2012
M104B: BELSORP-MAX X-ABAbsorption Measurement Instrument
  • ・Gas Adsorption/Desorption (He, N2) (CO2 is not available)
  • ・H2O, Organic liquids available
  • ・77 K (Liquid Nitrogen), 0~40 ˚C
  • ・BET surface area
  • ・Measurement in extremely low pressure
  • ・Pore distribution analysis
  • Installation Year: 2024
M201: Filgen Osmium Plasma Coater OPC-60AL
  • ・Making a conductive Os thin film on the surface of SEM samples for preventing charging. reducing granularity and heat damage
  • Installation Year: 2009
M202: JEOL Auto Fine Coater JFC-1600
  • ・Making a conductive Pt thin film on the surface of SEM samples for preventing charging and heat damage
  • Installation Year: 2009
M203: Vacuum Device Osumium Plasma Coater HPC-20
  • ・Making a conductive Os thin film on the surface of SEM samples for preventing charging. reducing granularity and heat damage
  • Installation Year: 2014

Nanohub base equipment being relocated to iCeMS

ImageName
C03: Liquid-Environment High Speed AFM
C08: Total Internal Reflection Fluorescence Microscope
C12: Optical Tweezers & 3D Trapping System