機器一覧 | iCeMS解析センター

画像	名称
	B001: ニコン高速共焦点顕微鏡 A1R ・Live-cell confocal imaging ・High-speed confocal imaging by resonant scanner and piezo z stage scanner ・4 laser lines (405, 488, 561, 640 nm) ・Spectral unmixing by 32ch spectral detector unit Installation Year: 2007 [ Detail ] ・Eclipse Ti-E, Inverted ・Ti PerfectFocus Unit (maintains the desired focal plane for long-term live cell imaging) ・Motorized XY Stage ・Piezo Z Scanner ・Epi Fluorescence Unit (Intensilight Precentered Fiber Illumination) ・DIC Unit ・Lasers 405, 488, 561, 640 nm ・Objectives CFI Plan Apo 10x, CFI Plan Apo VC 20x, CFI S Plan Fluor ELWD 20xC, CFI Plan Apo Lambda D 40x, CFI Plan Apo VC 60x oil, CFI Plan Apo VC 60x water ・Epi Fluorescence Filter Set　DAPI、FITC、TRITC ・High-speed Resonant Scanner (7.8 kHz) for High-speed Imaging at 30 fps (512 x 512 pixels) and 420 fps (512 x 32 pixels) ・2 x GaAsP Detectors (for 488 and 561 nm channels), 2 x PMT Detectors (for 405 and 640 nm channels), 1 x PMT Detector (for DIC) ・1 x 32 channels Spectral Detector Unit for Spectral Imaging at 1.67 fps (512 x 512 pixels) and 24 fps (512 x 32 pixels) ・Fluorescence Filters 450/50, 482/35, 525/50, 540/30, 595/50, 700/75 ・Cell Culture Chamber with Temperature and CO2 Control (Tokai Hit)
	B002: オリンパス共焦点超解像顕微鏡 FV-1000 ・Live-cell confocal imaging ・FV-OSR 2D super-resolution imaging with 120-nm (xy) resolutions ・6 laser lines (405, 440, 488, 515, 559, 635 nm) Installation Year: 2007 [ Detail ] ・Motorized Inverted Microscope with Automatic Focus Correction Unit IX81ZDC ・Motorized XY stage ・Epi Fluorescence Unit (Mercury Lamp Illumination) ・DIC Unit ・Lasers 405, 440, 488, 515, 559, 635 nm ・Objectives UPLSAPO 10x, UPLSAPO 20x, UPLSAPO 40x, UPLSAPO 60x OIL, UPLSAPO 100x OIL, UPLSAPO30XS Silicone Immersion ・Epi Fluorescence Filter Set DAPI, FITC, TRITC ・4 x PMT Detectors ・GaAsP Detector ・Super Resolution Unit and Software FV-OSR ・Cell Culture Chamber with Temperature and CO2 Control (Tokai Hit)
	B003: ライカ STED超解像顕微鏡 TCS SP8 STED ・Live-cell confocal imaging ・Gated STED 2D super-resolution imaging with 50-nm (xy) resolutions ・5 laser lines (458, 476, 488, 514, 633 nm) and white light laser (470 – 670 nm) ・1 to 3-color STED imaging by 2 depletion laser lines (592, 660 nm) Installation Year: 2009 [ Detail ] ・Motorized confocal microscope SP8 (based on Leica DMI 6000 CS) ・Adaptive Focus Control unit (maintains the desired focal plane for long-term imaging) ・Lasers Ar (458, 476, 488, 514 nm), HeNe (633 nm) White light laser: continuous wave laser in the range of 470-670nm; AOTF allows to choose up to 8 different wavelengths simultaneously; pulsed laser system with a repetition rate of 80 MHz Continuous wave depletion laser (592, 660 nm) for STED Ti:Sapphire pulsed laser (Coherent Chameleon) ・Detectors 2ch PMT fluorescence detectors 2ch high-sensitive hybrid GaAsP detector HyD; time gated detection allows improvement of STED lateral (xy) resolution to <50 nm by suppressing short-lived signals at the edge of focal spot 1ch Transmitted light detector ・Scanner FOV scanner with dual scanning galvanometer mirrors allows for either high speed scanning 7 images/sec at 512 x 512 or high pixel density scanning 8k x 8k (64 megapixels) per channel. ・Motorized XY stage and Z galvo stage for high-precision Z-sectioning ・DIC unit ・Objectives Dry lens HC PL APO CS 10x/0.40 Oil lens HCX PL APO CS 40x/1.25-0.75, HCX PL APO CS 63x/1.40-0.60, HC PL APO 100x/1.40 STED WHITE Water lens HCX PL APO 63x/1.20 W CORR CS ・Cell culture chamber with temperature and CO2 Control (Tokai Hit) ・Deconvolution software with STED option (Huygens)
	B004: ツァイス共焦点・FCCS顕微鏡 LSM780 ・Long-term live-cell confocal imaging ・7 laser lines (405, 440, 458, 488, 514, 561, 633 nm) ・Spectral unmixing by 32ch GaAsP detector array ・Molecular interaction detection by FCS/FCCS (ConfoCor3 with APD detector) Installation Year: 2009 [ Detail ] ・Motorized inverted microscope AxioObserver.Z1 ・Lasers 405, 440, 458, 488, 514, 561, 633 nm ・Detectors 34ch QUASAR GaAsP spectral detector 1ch Transmitted light detector 2ch　External detector for multiphoton (for GFP and RFP) 2ch APD detector for FCS/FCCS ・Automatic focus correction unit (Definite Focus) ・Motorized XY stage ・Wide-field fluorescence unit (X-Cite fiber-coupled light source) ・DIC unit ・Objectives Plan-Apochromat10x/0.45, Plan-Apochromat 20x/0.80, LCI Plan-Neofluor 25xImm/0.80, C-Apochromat 40xWater/1.20, Plan-Apochromat 63xOil/1.40 ・Cell culture unit (Incubation chamber + Heater + CO2 supply) ・Narishige micromanipulator
	B005: ツァイス超解像顕微鏡 LSM880 Airyscan ・Long-term live-cell confocal/super-resolution imaging ・6 laser lines (405, 458, 488, 514, 561, 633 nm) ・Airyscan super-resolution imaging with 120-nm (xy) and 350-nm (z) resolutions ・Airyscan fast mode with 4x acquisition speed ・Spectral unmixing by 32ch GaAsP detector array Installation Year: 2015 [ Detail ] ・Motorized inverted microscope AxioObserver.Z1 ・Lasers 405, 458, 488, 514, 561, 633 nm ・Detectors 34ch QUASAR GaAsP spectral detector 1ch　 Transmitted light detector Airyscan detector with fast acquisition mode (4x faster) ・Automatic focus correction unit (Definite Focus) ・Motorized XY stage ・Piezo Z stage ・Wide-field fluorescence unit (HXP120V fiber-coupled light source) ・DIC unit ・Objectives Plan-Apochromat10x/0.45, Plan-Apochromat 20x/0.80, LCI Plan-Neofluor 25xImm/0.80, Plan-Apochromat 40xOil/1.30, Plan-Apochromat 40xOil/1.40, Plan-Apochromat 63xOil/1.40 for super-resolution, Plan-Apochromat 100xOil/1.46 for super-resolution ・Cell culture unit (Incubation chamber + Heater + CO2 supply)
	B006: ツァイス共焦点顕微鏡 LSM710 ・Long-term live-cell confocal imaging ・5 laser lines (458, 488, 514, 561, 633 nm) ・Tiling/stitching for multiple fields Installation Year: 2009 [ Detail ] ・Motorized inverted microscope AxioObserver.Z1 ・Lasers 458, 488, 514, 561, 633 nm ・Detectors 2ch QUASAR spectral detector 2ch LSM BiG GaAsP detector (for GFP and RFP) 1ch Transmitted light detector ・Automatic focus correction unit (Definite Focus) ・Motorized XY stage ・Wide-field fluorescence unit (X-Cite fiber-coupled light source) ・DIC unit ・Objectives Plan-Apochromat10x/0.45, LD LCI Plan-Apochromat25xImm/0.80, Plan-Apochromat 40xOil/1.40, LD C-Apochromat 40xWater/1.10, Plan-Apochromat 100xOil/1.46 ・Cell culture unit (Incubation chamber + Heater + CO2 supply)
	B007: ツァイス多光子超解像高速顕微鏡 LSM980NLO Airyscan2 ・Long-term live-cell confocal/super-resolution/2-photon imaging ・7 laser lines (405, 445, 488, 561, 639, Chameleon Ultra II 680-1080 nm) ・Airyscan2 super-resolution imaging with 120-nm (xy) and 350-nm (z) resolutions ・Airyscan2 fast and gentle multiplex mode with 4x and 8x acquisition speeds ・Spectral unmixing by 32ch GaAsP detector array Installation Year: 2019 [ Detail ] ・Motorized inverted microscope AxioObserver.7 ・Lasers 405, 445, 488, 514, 561, 639 nm, Coherent Chameleon Ultra II (680-1080 nm) ・Detectors 34ch　 QUASAR GaAsP spectral detector 1ch　　Transmitted light detector Airyscan 2 detector with multiplex (4x and 8x) acquisition modes ・Automatic focus correction unit (Definite Focus.2) ・Motorized XY stage ・Piezo Z stage ・Wide-field fluorescence unit (HXP120V fiber-coupled light source) ・DIC unit ・Objectives Plan-Apochromat10x/0.45 Plan-Apochromat 20x/0.80 Plan-Apochromat 63xOil/1.40 for super-resolution alpha Plan-Apochromat 63xOil/1.46 for super-resolution ・Cell culture unit (Incubation chamber + Heater + CO2 supply) ・Arivis Vision 4D
	B008: アンドール高速共焦点顕微鏡統合システム Dragonfly ・Spinning disk confocal imaging with uniform Borealis illumination ・Fast live-cell imaging up to 400 fps and 3D imaging for large volume samples ・4 laser lines (405, 488, 561, 637 nm) ・2 high-sensitive cameras (iXon Ultra 888 EMCCD and Zyla 4.2 Plus sCMOS) ・Mosaic3 photostimulation unit with 405 nm laser Installation Year: 2019 [ Detail ] ・Dragonfly 502 Spinning disk with 2 pinhole diameters (25 and 40 microns), Borealis illumination, Laser-illuminated widefield mode, 2 camera ports, Camera magnification (3-position motorized), Illumination zoom, GPU-accelerated deconvolution in parallel with acquisition, Imaris Core ・Motorized inverted microscope Nikon Ti2-E ・Lasers 405 nm/100 mW, 488 nm/150 mW, 561 nm/100 mW, 637 nm/140 mW ・Cameras iXon Ultra EMCCD Zyla 4.2 Plus sCMOS　・Automatic focus correction unit (Perfect Focus) ・ASI motorized XY stage and stage piezo (500-micron range) ・Objectives CFI Plan Apochromat Lambda 10X Dry NA 0.45 WD 4.00 CFI Plan Fluor 20X MImm (Oil/Glycerin/Water) NA 0.75 WD 0.35-0.33 CFI Plan Apochromat Lambda S 25X Silicone NA 1.05 WD 0.55 CFI Plan Apochromat Lambda S 40X Silicone NA 1.25 WD 0.30 CFI Plan Apochromat VC 60XC Water NA 1.20 WD 0.31-0.28 CFI Apochromat TIRF 60XC Oil NA 1.49 WD 0.13-0.07 (37oC) CFI Apochromat TIRF 100XC Oil NA 1.49 WD 0.15-0.09 (37oC) CFI Plan Apo Lambda 60X Oil NA 1.40 WD 0.13 CFI Plan Apo VC 100X Oil NA 1.40 WD 0.13 ・Tokai Hit stage top incubator ・Mosaic3 photostimulation unit with 405 nm/450 mW laser
	B009: ツァイス格子構造化照明超解像顕微鏡 Elyra7 with Lattice SIM^2 ・Live-cell super-resolution imaging up to 255 fps ・4 laser lines (405, 488, 561, 642 nm) ・Light efficient lattice structured illumination imaging and advanced deconvolution (Lattice SIM^2) with 60-nm (xy) and 200-nm (z) resolutions ・SIM^2 Apotome mode for fast optical sectioning ・Simultaneous dual-color imaging by 2 high-sensitive cameras (PCO edge 4.2 sCMOS) Installation Year: 2021 [ Detail ] ・Motorized inverted microscope AxioObserver.7 ・Lasers 405 nm/50 mW, 488 nm/100 mW, 561 nm/100 mW, 642 nm/150 mW ・Cameras PCO edge 4.2 sCMOS x2 with Duolink module ・Automatic focus correction unit (Definite Focus.2) ・Motorized Z drive ・XY (130 x 100 mm) & Z (100 µm) piezo scanning stage ・Wide-field fluorescence unit (HXP120V fiber-coupled light source) ・Objectives EC Plan-Neofluar 10x/0.3 LD LCI Plan-Apochromat 25x/0.8 Imm Corr Plan-Apochromat 40x Oil/1.40 alpha Plan-Apochromat 100x Oil/1.46 ・Cell culture unit (Incubation chamber + Heater + CO2 supply)
	B101: BD フローサイトメーター Canto II ・two lasers (L1: Blue 488 nm, L2: Red 633 nm) ・six colors (See the following config for the details.) ・FACSDiva 8 software ・High Throughput Sampler (HTS), available for a 98- or 384-well microplate Installation Year: 2008 [ Detail ] Blue A: PE-Cy7 (735 LP, 780/60) Blue B: PerCP-Cy5-5 (655LP, 670LP) Blue C: (610LP) Blue D: PE (556LP, 585/42) Blue E: FITC (502LP, 530/30) Blue F: SSC (488/10) Red A: APC-Cy7 (735LP, 780/60) Red B: (685LP) Red C: APC (660/20)
	B102: BD セルソーター Aria II SORP ・4-lasers (L1: Blue 488 nm, L2: Red 640 nm, L3: UV 355 nm, L4: Green 532 nm) ・13 colors (See the following config for the details.) ・FACSDiva 8 software ・Plate sorting ・Cooling system Installation Year: 2009 [ Detail ] Blue A: PE-Cy7 (750 LP, 780/60) Blue B: PerCP-Cy5-5 (685LP, 710/50) Blue C: PE-Cy5 (635LP, 660/20) Blue D: PE-Texas Red (600LP, 610/20) Blue E: PE (550LP, 575/25) Blue F: FITC (505LP, 525/50) Red A: APC-Cy7 (750LP, 780/60) Red B: Alexa Fluor 700 (690LP, 730/45) Red C: APC (660/20) UV A: Hoechst Red (635LP, 670/50) UV B: Hoechst Blue (450/40) Green A: Grn PE-Cy7 (750LP, 780/60) Green B: Grn PE (575/25)
	B104: ProteinSimple キャピラリーイムノブロッティング Jess System ・Capillary chemiluminescent and fluorescent Western blotting ・2-40 kDa, 12-230 KDa or 66-440 kDa/8 x 13 or 8 x 25 capillary cartridge available Installation Year: 2020 [ Detail ] https://www.proteinsimple.com/jess.html メーカー提供サポート動画　(Movies in Japanese) 1. 測定編 (基本編)(前半10分は、概要説明) https://youtu.be/aFgkXrvO6EU 2. 解析編 (基本編) https://youtu.be/dtQMNhFyn7g 3. 検量線の書き方 https://youtu.be/uTzdYDM1mWI
	B105: Applied Biosystems 4キャピラリーDNAシーケンサー Seqstudio Genetic Analyzer ・Sanger sequencing ・4-capillary ・Thermo Fisher Connect compatible (available for remote-monitoring from home) Installation Year: 2020
	B106: Applied Biosystems RT-PCR装置 QuantStudio 3 ・three independent temperature zones for precise temperature control and optimization ・compatible with the standard 96 well (MicroAmp Optical 96-well Reaction Plate (Cat. no. N80 10560) or MicroAmp Optical 8-Tube strip (0.2 mL, Cat. no. 4316567)) ・Thermo Fisher Connect compatible (available for remote-monitoring from home) Installation Year: 2021
	M001: 日本電子電界放出形透過電子顕微鏡（FE-TEM） JEM-2200FS+JED-2300T Installation Year: 2009
	M002: 日本電子電界放出形走査電子顕微鏡（FE-SEM）JSM-7500F Installation Year: 2008
	M003: リガク X線吸収分析装置 XAS-Looper Installation Year: 2008
	M004: ブルカー核磁気共鳴装置（NMR）AVANCE-III 500USP Installation Year: 2009
	M006: 日本電子核磁気共鳴装置 (NMR) JNM-ECZ600R/M3 JNM-ECZ600R/M3 600MHz固体NMRシステム(24本ASC含む） CZR型固体NMR分光計（ミドルパワーアンプ搭載）＜プローブ＞・1 mm HX MAS ・広温域3.2 mm HX MAS ・8 mm HX MAS ・3.2 mm AutoMAS ・GR（高勾配磁場、固体用試料管含む） Installation Year: 2019
	M007: 日立ハイテクノロジーズショットキー走査電子顕微鏡　SU5000 Installation Year: 2014
	M103: 吸着装置 BELSORP-MINI X Installation Year: 2021
	M104: 吸着装置 BELSORP-MAX X Installation Year: 2010, 2012
	M201: フィルジェンオスミウムプラズマコーター OPC60AL Installation Year: 2009
	M202: 日本電子オートファインコーター JFC-1600 Installation Year: 2009
	M203: 真空デバイスオスミウムプラズマコーター HPC-20 Installation Year: 2014